Climate-associated variation in the drivers of benthic macroinvertebrate species-area relationships across shallow freshwater lakes.

The island species-area relationship (ISAR) describes how species richness increases with increasing area of a given island or island-like habitat, such as freshwater lakes. While the ISAR is one of the most common phenomena observed in ecology, there is variation in both the form of the relationship and its underlying mechanisms. We compiled a global data set of benthic macroinvertebrates from 524 shallow freshwater lakes, ranging from 1 to 293,300 ha in area. We used individual-based rarefaction to determine the degree to which ISAR was influenced by mechanisms other than passive sampling (larger islands passively sample more individuals from the regional pool and, therefore, have more species than smaller islands), which would bias results away from expected relationships between rarefied species richness (and other measures that capture relative abundances) and lake area. We also examined how climate may alter the shape of the ISARs. We found that both rarefied species richness (the number of species standardized by area or number of individuals) and a measure of evenness emphasizing common species exhibit shallow slopes in relationships with lake area, suggesting that the expected ISARs in these lakes most likely result from passive sampling. While there was considerable variation among ISARs across the investigated lakes, we found an overall positive rarefied ISAR for lakes in warm (i.e. tropical/subtropical) regions (n = 195), and in contrast, an overall negative rarefied ISAR in cool (i.e. north temperate) lakes (n = 329). This suggested that mechanisms beyond passive sampling (e.g. colonization-extinction dynamics and/or heterogeneity) were more likely to operate in warm lakes. One possible reason for this difference is that the area-dependent intensity of fish predation, which can lead to flatter ISARs, is weaker in warmer relative to cooler lakes. Our study illustrates the importance of understanding both the pattern and potential processes underlying the ISARs of freshwater lakes in different climatic regions. Furthermore, it provides a baseline for understanding how further changes to the ecosystem (i.e. in lake area or climate) might influence biodiversity patterns.

The role of bioturbation triggered by benthic macroinvertebrates in the effectiveness of the Floc & Lock technique in mitigating eutrophication.

Anthropogenic activities have led to excessive loading of phosphorus and nitrogen into water bodies, leading to eutrophication and promoting the growth of cyanobacteria, posing a threat to the health of humans and aquatic animals. Techniques such as Floc & Lock have been developed to mitigate eutrophication by reducing phosphorus concentrations in water and preventing algal blooms. However, little attention has been given to the impact of phosphorus resuspension by sediment-associated organisms such as benthic macroinvertebrates, on the effectiveness of this technique. Here, we experimentally evaluated whether the presence of snails Melanoides tuberculata (Müller, 1774) and larvae of Chironomus sancticaroli (Strixino and Strixino, 1981) affects the efficiency of the Floc & Lock technique. Snails and chironomid larvae are benthic macroinvertebrates commonly found in high abundance in eutrophic reservoirs. Specifically, we tested the hypotheses that (i) the presence of benthic macroinvertebrates reduces the efficiency of coagulants and clays in removing phosphorus and algal biomass from the water column, and (ii) this effect is species-dependent, as some organisms such as the snails, revolve the substrate and resuspend sedimented particles, while other ones, such as chironomid larvae, aid in the removal of phosphorus from the water column by depositing them in the sediment. Our findings revealed that the impact of benthic macroinvertebrates on the effectiveness of the Floc & Lock technique is species-dependent. Chironomid larvae positively influenced the efficiency of the technique by aiding in the removal of total phosphorus, soluble reactive phosphorus, and algal biomass from the water column, depositing them in the sediment. In contrast, the presence of snails had the opposite effect, resulting in increased phosphorus concentration and algal biomass in the water. Surprisingly, the snails consumed the flocs formed by the coagulant and clay within a short time interval of 72 h, raising concerns due to the presence of toxic cyanobacterial biomass in these flocs. Our study emphasizes the importance of considering benthic macroinvertebrates and their impact on the effectiveness of eutrophication management techniques.

Functional threshold responses of benthic macroinvertebrates to environmental stressors in reservoirs.

Reservoirs are aquatic ecosystems created by humans to supply water needs. They can impair aquatic diversity due to the lack of connectivity, reduced water volume, and pressures exerted by surrounding human activities. These changes are expected to produce abrupt fluctuations in the reservoirs' environment, thus influencing the structure and functioning of aquatic communities. Therefore, this study aimed to understand the impact of a range of environmental stressors in reservoirs on benthic macroinvertebrates by analyzing their functional threshold response. Biological data were collected in six reservoirs from the semi-arid region of Northeast Brazil, as case study. A total of 37.874 benthic macroinvertebrates belonging to 35 taxa were collected. Nevertheless, almost 90% of this abundance belonged to three species alone, considered generalists, with multivoltine reproduction and from the gatherer-collectors feeding group. Increases in environmental stressors such as salinity, nitrate, ammonia, and dissolved solids led to the selection of macroinvertebrates with specific traits (e.g., protected body, gill respiration, and large body size). These functional traits showed differences in their threshold response depending on the stressors and are indicators of the effects of these stressors on the reservoirs. Some of the potential sensitive traits (with a negative threshold response to the stressor) could also associate with other stressors, demonstrating that tolerance of benthic macroinvertebrates is defined by a set of functional characteristics. Overall, the increase in stressor' gradients selected functionally tolerant organisms with high resistance capacity, but these were represented by dominant species. This resulted in low diversity in the reservoirs, which may compromise ecosystem functioning, and raises concerns about adequate management of the systems.

Impacts of the Floc and Sink technique on the phytoplankton community: A morpho-functional approach in eutrophic reservoir water.

The Floc and Sink technique promotes, through the application of coagulants and clays, the removal of phosphorus and algal biomass from the water column by flocculation and sedimentation. Although it is a promising technique for mitigating harmful cyanobacterial blooms, little is known about the impacts on other phytoplankton species as well as it is not known how species with mechanisms of resistance to sedimentation respond to the application of these products. In this study, a laboratory experiment was carried out with water from a eutrophic reservoir to assess the impact of applying aluminum-based coagulants, aluminum sulfate and polyaluminium chloride, and chitosan, alone and combined with lanthanum modified bentonite and natural bentonite on the phytoplankton community, in a functional approach based on morphology (Morphology-Based Functional Groups - MBFG, Kruk et al., 2010 and Reynolds et al., 2014), with an emphasis on the characteristics that provide resistance to sedimentation. We tested two hypotheses: phytoplankton species with adaptive mechanisms that provide buoyancy to cells are more resistant to the removal from the water column by coagulants and clays; and coagulants based on metals and modified clays are more efficient in sedimentation of microalgal cells compared to natural products, regardless of the presence of an adaptive mechanism of resistance to sedimentation. Our results showed that aluminum sulfate and polyaluminium chloride alone or combined with lanthanum modified bentonite and natural bentonite effectively sedimented the cells, regardless of the presence of buoyancy mechanisms. In contrast, the natural coagulant chitosan alone or combined with lanthanum modified bentonite and natural bentonite removed only those species that were small-celled or small colonial without specialized structures or with the presence of spines, arms and siliceous exoskeleton. In the case of species with aerotopes and flagella, the removal was not effective and still caused an increase in algal biomass due to the formation of suspended cell aggregates on the surface of the water column of the experimental units. Therefore, we concluded that the Floc and Sink technique has an impact on the phytoplankton community because it removes from the water column species that are not the target of coagulants and clays, but that are considered important sources of energy in freshwater trophic webs. This result differs according to the type of product used as well as it is related to the morphological adaptations that favor the buoyancy of cells in the water column. Species with aerotopes and flagella are more resistant to sedimentation and may make the use of products applied in the Floc and Sink technique unfeasible. Nevertheless, these results represent only an immediate effect of the technique on the phytoplankton community, thus requiring a longer time scale evaluation to determine the algae that can effectively recover water column. Therefore, we also emphasize that algae of nutritional value can recover over time and make the long-term application of the technique acceptable.

Perception of the local community: What is their relationship with environmental quality indicators of reservoirs?

Evaluating the conservation of aquatic ecosystems, especially those which serve to supply, has been carried out using a variety of tools. However, the perception of water quality by the local community which lives in direct contact with water resources has not been considered with enough importance. This study analysed the relationship between the conservation status of reservoirs as perceived by the local community and their conservation status according to physical, chemical, and biological indicators. To do so, we calculated the Trophic State Index (TSI) of the reservoirs, the diversity and richness of benthic macroinvertebrate and we analysed the human influence in the riparian zone. Thus, we created the Community Conservation Perception Index (CCPI) to quantitatively associate the perception of the local community with environmental quality indicators (TSI, diversity, richness and anthropogenic influences in the riparian zone). We found that interviewee perception of reservoir conservation (using the CCPI) was related to trophic state, richness and diversity of benthic macroinvertebrate, and the presence of residences and agriculture in the riparian zone. It is necessary to consider the environmental perception of the local community as a relevant factor in management programmes and the conservation of ecosystems, even if artificial, as is the case with reservoirs. These communities can significantly contribute to maintaining the environmental quality through their performance in participatory management in projects such as: participating in the investigation of pollution in reservoirs, collecting parameters related to water quality, and community action in designing conservation strategies.

Extreme drought scenario shapes different patterns of Chironomid coexistence in reservoirs in a semi-arid region.

Semi-arid regions are particularly prone to extreme climate events such as droughts, which result in drastic fluctuations in the water volume of aquatic ecosystems, including artificial ones. As these climate extremes intensify, species must adapt, however, not all species can persist under new climate regimes in such a short period of time. In this study, we evaluated how fluctuations in the water levels of reservoirs, caused by drought, affect Chironomidae diversity patterns in a semi-arid region. We studied six reservoirs (256 sites) in two basins in Northeastern Brazil, exposed to different levels of anthropic impact. Sampling was carried out in 2014, 2015 (both extremely dry years) and 2019. A dead water volume was attained during the extreme drought in 2015, consequently affecting the reservoir and resulting in a low diversity, abundance, and functional redundancy of the Chironomidae assemblages. Despite precipitation increases in 2019, some reservoirs continued to be water deficient. These drastic water fluctuations led to different patterns in Chironomidae taxonomic and functional diversity, which were also influenced by anthropic stressors. Thus, the most impacted basin presented lower diversity, with some species and trait turnover between reservoirs. The opposite trend was observed in the least impacted basin. Overall, taxonomic and functional diversity decreased with decreasing water volume, resulting in a community dominated by small-medium sized individuals with multivoltine cycles and hemoglobin and diapause resistant forms, conferring higher tolerance to water stress. The drought and consequent water volume fluctuations throughout the years seemed to exacerbate the water quality due to pre-existing exposure to anthropic impacts (e.g., domestic discharge, fishing activity, agriculture, livestock). This resulted in biotic homogenization, with an observed loss of taxa and traits. This study reinforced the need to implement habitat conservation and water quality improvement strategies to prevent further ecosystem damage in the face of climate change uncertainty.

Performance of richness estimators for invertebrate inventories in reservoirs.

Biological inventories combined with the estimation of species richness represent a useful tool for the analysis of the pattern of species distribution in different regions. This study aimed to (i) comparatively evaluate the performance of non-parametric richness estimators for invertebrate inventories in reservoirs between ecoregions and (ii) to assess whether the efficiency (bias, precision and accuracy indices) of the estimators is altered when applied to sites from different ecoregions. The study was conducted in the ecoregions Central Pediplano of the Borborema Plateau (Paraíba River basin) and Northern Sertaneja Depression (Piranhas-Assu River basin), semiarid region of Brazil. Six reservoirs were selected and benthic macroinvertebrates were sampled at 141 sites distributed along the littoral zone, in 4 periods (June, September, December 2014 and March 2015). The organisms were identified to the family level, except for Chironomidae, identified to the genus level. We comparatively analyzed six non-parametric richness estimators: Jackknife 1, Jackknife 2, Chao1, Chao 2, ICE, and Bootstrap, and three performance indicators: bias, precision, and accuracy. ICE and Jackknife 2 had more stable results for total species richness, but with different performance between ecoregions for bias, precision, and accuracy. Variation in performance of the estimators may be associated with differences in species richness and frequency between ecoregions. ICE and Jackknife 2 proved to be the best estimators for biological inventories of aquatic invertebrates in reservoirs in studies comparing data from different ecoregions, due to accuracy and precision, while Bootstrap is the least indicated, given greater bias and less accuracy and precision.

Removal efficiency of phosphorus, cyanobacteria and cyanotoxins by the "flock & sink" mitigation technique in semi-arid eutrophic waters.

Geoengineering techniques have been used to control phosphorus and cyanobacteria in lakes promising greater and quicker chemical and ecological recovery. Techniques that use coagulants and clays to remove particulates and dissolved phosphorus from the water column have received great. In this study, bench-scale "flock & sink" assays were carried out to evaluate the efficiency of the coagulants aluminium sulphate (SUL), polyaluminium chloride (PAC) and chitosan (CHI), alone and combined with natural bentonite clays (BEN) and lanthanum-modified bentonite (LMB), to remove of phosphorus from a eutrophic reservoir in a semi-arid region of Brazil. In addition, the study seeks to assess the effects on the cyanobacteria density and the intra- and extracellular concentrations of cyanotoxins after the application of these geoengineering materials. The SUL and PAC coagulants effectively reduced the total phosphorus (TP), reactive soluble phosphorus (SRP), turbidity, chlorophyll-a, cyanobacteria density and intracellular microcystin, whereas CHI showed a low removal efficiency. Lanthanum-modified bentonite proved to be more effective than BEN; however, the application of the coagulants only was sufficient to successfully remove phosphorus and cyanobacteria from the water column. In addition, the efficiency of the "flock & sink" technique in cell removal varied among the cyanobacteria species. Small colonial species such as Aphanocapsa delicatissima, Merismopedia glauca and Merismopedia tenuissima were removed regardless of the treatment used, including those with CHI and BEN. As for the filamentous cyanobacteria, Cylindrospermopsis raciborskii, Geitlerinema amphibium, Planktothrix agardhii and Pseudanabaena catenata, removal was achieved only using PAC, SUL and LMB alone or when combined. The intracellular concentrations of saxitoxin and cylindrospermopsin and the extracellular fraction of these cyanotoxins and of microcystin were not influenced by the application of coagulants and clays. This indicates that cell lysis did not occur with the addition of the geoengineering materials. These results demonstrate that the "flock & sink" technique could be used for restoration of eutrophic waters.

Effectiveness of abundance and biomass curves in detecting environmental alterations in semi-arid region reservoirs / Eficácia das curvas de abundância e biomassa na detecção de alterações ambientais em reservatórios do semiárido

Abstract For mitigation of environmental problems generated by bad water resource management, the first step is to develop methods for effective diagnosis. The potential bioindicator, mainly in the benthic community, in the evaluation of water quality is the structure of the community, associated with magnitude of environmental impact. The aim of the present study was to evaluate the effectiveness of abundance biomass curves (ABC) as tools to estimate ecological quality in reservoirs in the Brazilian semi-arid region. Two reservoirs, Epitácio Pessoa and Argemiro de Figueiredo were selected. These reservoirs are located in the watershed of the Paraíba River, in Northeast Brazil. Sampling was done at 40 sites in the littoral region of each reservoir, during the periods of higher and lower water volume in the reservoirs. ABC for semi-arid region reservoirs showed disparate results for biological and environmental indicators, because they had a better relationship with momentary variables (physical and chemical parameters). This may be related to the high occurrence of exotic species, principally Corbicula largillierti, which has a high biomass, in association with a severe drought period. ABC must thus be used with parsimony and combined with other indicators, for an accurate and coherent characterization.

Resumo Para mitigação de problemas ambientais gerados pela má gestão de recursos hídricos, o primeiro passo é o desenvolvimento metodologias de diagnósticos eficientes. O potencial bioindicador, principalmente da comunidade bentônica, na avaliação da qualidade da água deve-se aos aspectos estruturais dessa comunidade, associado com a magnitude de impactos ambientais. O objetivo do presente estudo foi avaliar a eficiência das curvas de biomassa e abundância (ABC) como ferramentas para estimar a qualidade ecológica em reservatórios na região do semi-árido brasileiro. Dois reservatórios, Epitácio Pessoa e Argemiro de Figueiredo foram selecionados. Estes reservatórios estão localizados na bacia do rio Paraíba, no nordeste do Brasil. As coletas foram realizadas em 40 locais na região litorânea de cada reservatório, durante os períodos de maior e menor volume de hídrico. As curvas ABC, quando plotadas em reservatórios do semiárido, apresentaram resultados diferentes aos indicadores biológicos e ambientais, tendo uma relação melhor com as variáveis ​​momentâneas (parâmetros físicos e químicos). Isso pode estar relacionado à alta ocorrência de espécies exóticas, principalmente Corbicula largillierti, o que reflete uma elevada biomassa, combinada com um período severo de seca. O método ABC devem ser utilizadas com parcimônia e associada a outros indicadores, para uma caraterização coerente e precisa.

Effects of an atypical drought on the benthic macroinvertebrate community in a tropical reservoir / Efeitos de uma seca atípica sobre a comunidade de macroinvertebrados bentônicos em um reservatório tropical

Abstract Atypical drought events have increasingly occurred in Brazil over the last years due to global climate changes. However, their consequences on aquatic biota in reservoirs are poorly known. We tested the hypothesis that macroinvertebrate communities are negatively affected by atypical drought events, given the sensitivity of many taxa to environmental changes. We predicted that: (a) there would be changes in limnological and sediment parameters between a regular year and an atypical year, (b) abundance and richness of the genera of Chironomidae and of exotic species would be higher due to the enhanced ability of these organisms to adapt to changes in the physical environment, and (c) community structure metrics (i. richness; ii. % richness; iii. abundance; iv. % abundance) would be affected by disturbance indices (i. Buffer Disturbance Index-BDI; ii. Local Disturbance Index-LDI; iii. Integrated Disturbance Index-IDI) in both years. The study was carried out in the reservoir of the Nova Ponte Hydroelectric Power Plant, state of Minas Gerais, comparing two sampling periods: a regular climatological year (2010) and an atypical drought year (2014). A total of 40 sampling sites were defined along the shore of the reservoir, and types of land use in the surrounding area of each site were measured, as well as physical habitat conditions, sediments, and benthic macroinvertebrate communities. Sampling was performed at these sites in the end of the rainy season in both years. The intensity of anthropogenic modifications was assessed at local scale and in the areas (buffers) surrounding the sampling sites using quantitative disturbance indices. There were striking differences in limnological parameters and sediment characteristics between sampling periods. Taxonomic richness was significantly lower in the drought year. As opposed to our predictions, richness and abundance of Chironomidae and exotic species did not increase with the atypical drought event. Besides, most community structure metrics showed a significant relationship with disturbance indices only during the regular climatological year, thus indicating that the large-scale effects of water stress may override the conditions of local habitats and the surrounding landscape. Therefore, in addition to a correct political-environmental management of water resources at local-scale, which includes maintaining the water quality and the riparian and landscape integrity, addressing large-scale climate issues is required for the maintenance of the ecological integrity of tropical reservoirs.

Resumo A ocorrência de episódios atípicos de seca vem crescendo nos últimos anos no Brasil em decorrência de mudanças globais no clima. Entretanto, as consequências disso para a biota aquática são ainda pouco conhecidas. Testamos a hipótese de que comunidades de macroinvertebrados são negativamente afetadas por eventos de seca atípica, dada a sensibilidade de muitos taxa às alterações ambientais. Nossas predições foram que (a) parâmetros limnológicos e características de sedimento seriam alterados em um ano de seca atípica, (b) encontraríamos maior abundância e riqueza de gêneros de Chironomidae e de espécies exóticas dada à alta capacidade destes organismos de adaptarem-se às mudanças no ambiente, (c) métricas de estrutura de comunidade (i. riqueza; ii. % riqueza; iii. abundância e iv. % abundância) seriam afetadas por índices de distúrbio (i. Índice de Distúrbio no Buffer-IDB; ii. Índice de Distúrbio Local-IDL e iii. Índice de Distúrbio Integrado-IDI) em ambos os anos. O estudo foi realizado no reservatório da Usina Hidrelétrica de Nova Ponte, Minas Gerais, comparando dois períodos amostrais: um ano climatológico normal (2010) e um ano de seca atípica (2014). Foram definidos 40 sítios amostrais na região litorânea do reservatório onde foram mensurados os tipos de usos do solo no entorno, condições de habitats físicos, características limnológicas na coluna d'água, sedimento e comunidades de macroinvertebrados bentônicos. A intensidade de alterações antrópicas foi avaliada em escala local e no entorno dos pontos amostrais (buffers) por meio de índices de distúrbio. Observamos diferenças expressivas nos parâmetros limnológicos e nas características dos sedimentos entre os dois períodos amostrais. A riqueza taxonômica foi significativamente menor no ano de seca. Ao contrário do esperado, os valores de riqueza e abundância de Chironomidae e de espécies exóticas não aumentaram com o evento extremo de seca. Paralelamente, a maioria das métricas de estrutura de comunidade apresentou relação significativa com os índices de distúrbio somente durante o ano climatológico normal, indicando que os efeitos de estresse hídrico em larga escala podem se sobrepor às condições locais de habitat e de paisagem circundante. Portanto, deve ser considerada não só a correta gestão político-ambiental de recursos hídricos em escala local, que inclui a manutenção da qualidade da água e da integridade da região ripária e uso do solo da região de entorno, mas também abordar questões climáticas em larga escala faz-se necessário para a manutenção da integridade ecológica em reservatórios tropicais.

How do people gain access to water resources in the Brazilian semiarid (Caatinga) in times of climate change?

Climate change is becoming an imminent reality, especially in arid and semiarid regions. Therefore, it is essential to understand the relationships between humans and aquatic ecosystems in order to devise efficient management and conservation strategies. We conducted 126 interviews using a semi-structured form to record water sources, transport strategies, and the use and treatment of water by communities surrounding four reservoirs within two drainage basins in the semiarid region of Brazil. These factors were then compared to the mean water volumes of the respective reservoirs from 2013 to 2015, a period of severe drought in that area. Seven types of water sources were considered, according to the perspectives of the interviewees: large reservoirs (dams) (43% of the citations), other smaller reservoirs (25%), rainwater (17.5%), wells (7%), waterholes (3%), bottled water (4%), and water tanks (0.5%). The water resources obtained are transported to human residences in seven different manners: actively pumped (34% of the citations), by water tanker truck (33%), distributed in pipes by local resident associations (11%), transport by animal (14%), human transport (4%), by car (2%), and by motorcycle (2%). The water is then used for domestic purposes (21%), for personal hygiene (20%), by animals (19%), in agriculture (18%), for cooking (10%), for fishing (7%), and for drinking (6%). A worrisome trend was that many local residents did not treat the water they were consuming. Climate change affects seasonal patterns of rainfall that will, in turn, determine the availability and quantities of water resources, provoking changes in the sources of water used by human populations, their strategies of access to that resource, and water-use patterns. It is necessary sustainable use of water resources based on the realities of local populations.

Application of a statistical model for the assessment of environmental quality in neotropical semi-arid reservoirs.

The aim of this study was to develop a statistical model to assess the environmental quality of reservoirs located in semi-arid region using metrics of anthropogenic disturbance, water quality variables, and benthic macroinvertebrate communities as indicators. The proposed model was applied to 60 sites located in three reservoirs in the Paraíba river basin, Brazilian semi-arid region. Collections were made in December 2011. In each site, we collected one sample of benthic macroinvertebrates and one water sample for the determination of physical and chemical parameters. Characterization of the landscape was made through application of 10 physical habitat protocols on each site for the collected information on disturbance and subsequent calculation of disturbance metrics. The results showed the formation of two groups: group 1, consisting of 16 minimally altered sites, and group 2, with 44 severely altered sites. The proposed statistical model was sensitive enough to detect changes. In the minimally altered group, the Chironomids Aedokritus and Fissimentum were dominant, indicating a higher environmental quality, while Coelotanypus and Chironomus were abundant in severely altered sites with lower environmental quality. The conservation and management of reservoirs in semi-arid regions should be intensified in view of the need to maintain the environmental quality of these ecosystems.

Assessment of disturbance at three spatial scales in two large tropical reservoirs.

Large reservoirs are an increasingly common feature across tropical landscapes because of their importance for water supply, flood control and hydropower, but their ecological conditions are infrequently evaluated. Our objective was to assess the range of disturbances for two large tropical reservoirs and their influences on benthic macroinvertebrates. We tested three hypotheses: i) a wide variation in the level of environmental disturbance can be observed among sites in the reservoirs; ii) the two reservoirs would exhibit a different degree of disturbance level; and iii) the magnitude of disturbance would influence the structure and composition of benthic assemblages. For each reservoir, we assessed land use (macroscale), physical habitat structure (mesoscale), and water quality (microscale). We sampled 40 sites in the littoral zones of both Três Marias and São Simão Reservoirs (Minas Gerais, Brazil). At the macroscale, we measured cover percentages of land use categories in buffer areas at each site, where each buffer was a circular arc of 250 m. At the mesoscale, we assessed the presence of human disturbances in the riparian and drawdown zones at the local (site) scale. At the microscale, we assessed water quality at each macroinvertebrate sampling station using the Micro Disturbance Index (MDI). To evaluate anthropogenic disturbance of each site, we calculated an integrated disturbance index (IDI) from a buffer disturbance index (BDI) and a local disturbance index (LDI). For each site, we calculated richness and abundance of benthic macroinvertebrates, Chironomidae genera richness, abundance and percent Chironomidae individuals, abundance and percent EPT individuals, richness and percent EPT taxa, abundance and percent resistant individuals, and abundance and percent non-native individuals. We also evaluated the influence of disturbance on benthic macroinvertebrate assemblages at the entire-reservoir scale. The BDI, LDI and IDI had significantly greater average scores at São Simão than at Três Marias Reservoir. The significantly greater differences in IDI scores for São Simão Reservoir were reflected in 10 of the 13 Ekman-Birge dredge biotic metrics and in 5 of 13 of the kick-net biotic metrics. We also observed clear ranges of disturbances within both reservoirs at macro (BDI) and mesoscales (LDI) and in water quality, but an insignificant range in MDI results. However, we found no significant relationship between the benthic macroinvertebrate metrics and the BDI, LDI, and IDI among sites within a single reservoir. Hence, we believe that benthic macroinvertebrate distributions in those reservoirs were influenced by other factors or that reservoir macroinvertebrates (dominated by chironomids) were poor indicators of disturbance at the site scale.

Tropical Estuarine Macrobenthic Communities Are Structured by Turnover Rather than Nestedness.

Turnover (i.e., species substitution) and nestedness (i.e., subsets of species from more diverse locations), the two main mechanisms used to explain the beta diversity of biological communities, have different implications for biodiversity conservation. To better understand how these mechanisms contribute to beta diversity, we tested the following hypotheses: (i) greater dissimilarity in community composition occurs between estuarine zones than other hierarchical level studied; (ii) beta diversity in these communities develops by turnover in estuaries with a lower degree of anthropogenic impact, but by nestedness in estuaries with a greater degree of anthropogenic impact; and (iii) the structuring mechanism is independent of season. We studied two tropical estuaries (dry and wet seasons) that vary in terms of land-use of the drainage basins. Subtidal benthic macroinvertebrates were sampled along the estuarine gradient in each of the two estuaries. The additive partitioning approach to species diversity was used to determine the hierarchical scale with the greatest dissimilarity in community composition. General beta diversity was measured using the Sorensen dissimilarity index, partitioning the turnover and nestedness components. The greatest dissimilarity in the composition of the communities occurred between the zones along the estuarine gradient in both seasons (dry = 58.6%; wet = 46.3%). In the estuary with a lower degree of anthropogenic influence, benthic macroinvertebrate diversity was generated by turnover regardless of the season. In the estuary with a greater degree of anthropogenic impact, beta diversity was structured by turnover during the dry season and a combination of both mechanisms during the wet season. We conclude that turnover is the principal mechanism responsible for beta diversity in benthic macroinvertebrate communities in tropical estuaries.

Potential ecological distribution of alien mollusk Corbicula largillierti and its relationship with human disturbance in a semi-arid reservoir / Potencial ecológico de distribuição do molusco alienígena Corbicula largillierti e sua relação com perturbação humana em um reservatório no semiárido

The introduction of exotic mollusk species has resulted in loss of biodiversity in semi-arid neotropical aquatic ecosystems. This study aims to relate the presence and density of Corbicula largillierti species to human disturbance, providing data for the conservation and management of aquatic ecosystems. Specimens were collected at Epitácio Pessoa reservoir in December 2011 and June 2012, presenting densities of 20.96 and 62.89 individuals per square meter, respectively. Anthropic disturbance metrics were calculated considering the presence, type and intensity of disturbance in littoral, riparian and flood zones. The occurrence of C. largillierti was mainly associated to the variables total phosphorus (261.05 ± 342.22 µg/L) and total nitrogen (440.79 ± 103.77 µg/L), near to tributaries. The occurrence of exotic mollusk species is evidence of the need for freshwater ecosystem conservation and management, particularly in reservoirs used for water supply.

A introdução de espécies exóticas de moluscos resulta em perda de biodiversidade em ecossistemas aquáticos semiáridos neotropicais. Este estudo tem o objetivo de relacionar a presença e densidade da espécie Corbicula largillierti ao distúrbio humano, fornecendo dados para a conservação e manejo de ecossistemas aquáticos. Os espécimes foram coletados no reservatório Epitácio Pessoa em dezembro de 2011 e junho de 2012, com densidades de 20,96 e 62,89 indivíduos por metro quadrado, respectivamente. Métricas de perturbação antrópica foram calculadas considerando a presença, tipo e intensidade de perturbação nas zonas litorânea, ribeirinha e de inundação. A ocorrência de C. largillerti esteve associada ès variáveis fósforo total (261,05 ± 342,22 μg/L) e nitrogênio total (440,79 ± 103,77 μg/L), em locais próximos aos afluentes. A ocorrência de espécies exóticas de moluscos evidencia a necessidade de conservação e manejo de ecossistemas de água doce, principalmente em reservatórios utilizados para abastecimento de água.

Ecological assessment of a southeastern Brazil reservoir / Avaliação Ecológica de um reservatório no sudeste do Brasil

Reservoirs are artificial ecosystems with multiple functions having direct and indirect benefits to humans; however, they also cause ecological changes and influence the composition and structure of aquatic biota. Our objectives were to: (1) assess the environmental condition of Nova Ponte Reservoir, Minas Gerais state, southeastern Brazil; and (2) determine how the aquatic biota respond to disturbances. A total of 40 sites in the littoral zone of the reservoir were sampled to characterize physical and chemical habitat, land use, and benthic macroinvertebrate and fish assemblages. The predominant type of land cover near the reservoir was natural vegetation. A total of 29 fish species and 39 macroinvertebrate taxa were collected, including eight alien species. Most sites had intermediate levels of human disturbance, however, high levels of degradation were associated with high proportions of alien species. Disturbances at multiple scales may alter natural patterns and processes, leading to environmental changes and damaging biological communities. Our results reinforce the importance of assessing reservoir ecological conditions at several scales. The study of land use, littoral zone physical habitat characteristics, water quality, and assemblage structure set the ground for proposing actions to rehabilitate and conserve aquatic ecosystems.

Reservatórios são ecossistemas artificiais com múltiplas funções e que oferecem benefícios diretos e indiretos ao homem; no entanto, também causam mudanças ecológicas e influenciam a composição e estrutura da biota aquática. Os objetivos deste estudo foram: (1) avaliar a condição ambiental do reservatório de Nova Ponte, Estado de Minas Gerais, sudeste do Brasil; e (2) verificar se a biota aquática responde aos distúrbios. No total, 40 sítios amostrais na região litorânea do reservatório foram amostrados para caracterizar os habitats físicos e químicos, cobertura e uso do solo, assembleias de macroinvertebrados e peixes. Vegetação natural de cerrado foi o tipo predominante de cobertura do solo no entorno do reservatório. 29 espécies de peixes e 39 taxa de macroinvertebrados bentônicos foram coletadas, incluindo oito espécies exóticas. A maioria dos sítios amostrais possui níveis intermediários de distúrbio antrópico, porém o aumento de alterações antrópicas foi associado ao aumento do número e abundância de espécies exóticas. Distúrbios em múltiplas escalas podem alterar os padrões e processos naturais, levando a mudanças ambientais e nas comunidades biológicas. Nossos resultados reforçam a importância de avaliar as condições ecológicas do reservatório em várias escalas. O estudo do uso e cobertura do solo, características de habitats físicos na zona litorânea, características limnológicas e estrutura das assembléias fornecem bases para a proposição de ações para reabilitação e conservação de ecossistemas aquáticos.

First record of Corbicula largillierti (Philippi 1844) in the Paraíba River Basin and potential implications from water diversion of the São Francisco River / Primeiro registro de Corbicula largillierti (Philippi 1844) na bacia do Rio Paraíba e implicações potenciais com a transposição das águas do Rio São Francisco

Corbicula largillierti is a native mollusk from China. In Brazil, this species was first recorded in the Pantanal of Mato Grosso. This short communication reports the occurrence of C. largillierti for the first time in the Paraíba river basin (Brazilian semi-arid), and also considers the risk of introduction of other molluscs invaders in this basin due to the diversion of water from the São Francisco River. Densities of individuals ranged from 33 to 65 ind.m-2 (maximum values of 484 ind.m-2) in coarse sediment (gravel, 2-4 mm). The diversion of waters from the São Francisco river can lead to the introduction of new species, enhancing ecological problems in the Paraiba river basin.

Corbicula largillierti é um molusco nativo da China. No Brasil esta espécie foi registrada primeiramente no Pantanal do Mato Grosso. Esta nota registra a primeira ocorrência de C. largillierti na bacia do Rio Paraíba (semiárido brasileiro). Considera também os riscos potenciais de introdução de outros moluscos invasores nesta bacia devido è transposição das águas do Rio São Francisco. As densidades do molusco variaram de 33 a 65 ind.m-2 (atingindo valor máximo de 484 ind.m-2) em sedimentos grossos (cascalho, 2-4 mm). A transposição das águas do Rio São Francisco pode ocasionar a introdução de novas espécies exóticas potencializando problemas ecológicos na bacia do Rio Paraíba.

Maximum ecological potential of tropical reservoirs and benthic invertebrate communities.

The Reference Condition Approach (RCA) is now widely adopted as a basis for the evaluation of the ecological quality of water bodies. In accordance with the RCA, the integrity of communities found in a given location should be analyzed according to their deviation from the communities that would be expected in the absence of anthropogenic disturbances. The RCA was used here with the aim of defining the Maximum Ecological Potential (MEP) of tropical reservoirs located in the hydrographical basin of the Paraopeba River in the state of Minas Gerais, Brazil. Among the reservoirs, Serra Azul is used as a water supply and is located in a core area of environmental protection where tourism is not allowed and the native vegetation is conserved. The benthic macroinvertebrate communities at 90 sites located in three reservoirs were analyzed and sampled every 3 months over 2 years. The temporal patterns of the communities in the three reservoirs were analyzed (2nd-STAGE MDS and ANOSIM) and were not significantly related to seasonal fluctuations in temperature and precipitation. Twenty-eight sites belonging to the Serra Azul reservoir were selected to define the MEP of these reservoirs because these sites had the lowest human disturbance levels. The macroinvertebrate taxa present in the selected MEP sites are similar to those of natural lakes and different from the communities of disturbed sites. The biological classification of these sites revealed two groups with distinct macroinvertebrate communities. This distinction was related to climatic variables, bottom substrate type, the presence of gravel/boulders, coarse sand, silt, clay or muck, depth, and the shoreline substrate zone. These two subsets of biological communities and respective environmental conditions can serve as a basis for the future implementation of ecological quality monitoring programs for tropical reservoirs in the study area. This approach can also, however, be implemented in other geographic areas with artificial or heavily modified water bodies.